High-resolution mass spectrometry: Theoretical and technological aspects

Over the past decade, time-of-flight (TOF) and Orbitrap™ (Thermo Fisher Scientific) mass spectrometers have aroused a growing interest, we observed progressive replacement of low-resolution (LRMS) by high-resolution (HRMS). Thanks to high accuracy, they provide an unambiguous identification allow structural elucidation unknown compounds. Furthermore, offer new powerful acquisition modes based on untargeted screenings dependent or independent previous information. These advances are particular interest in field toxicology, increasing number HRMS presentations at scientific meetings, corresponding original papers, review articles dealing with application HRMS. The aim this study is compare respect LRMS detail explain physical technological principles which TOF Orbitrap analyzers based, data processing methods. Finally, three applications toxicology detailed highlight technology toxicology.